What is multiplex PCR? - Front Line Genomics
WEBJul 26, 2021 · Multiplex PCR is a technique whereby PCR is used to amplify several different DNA sequences simultaneously. It is a type of target enrichment approach. It was first described in 1988 as a method to detect deletion mutations in the dystrophin gene – the largest known human gene. The dystrophin complex connects the …
Sample Preparation for NGS – A Comprehensive Guide
WEBAug 21, 2024 · Step #3: Amplification. This is an optional step, but it is usually required. It is dependent on the application of NGS and the sample size. Amplification becomes essential to obtain enough coverage for reliable sequencing for samples with small amounts of starting material.
Long-read sequencing vs short-read sequencing
WEBJul 26, 2021 · Long-read sequencing. Long-read sequencing technologies are capable of reading longer lengths, between 5,000 and 30,000 base pairs. Therefore, they immediately address one of the main challenges faced by short-read sequencing. They sequence a single molecule, eliminating amplification bias, and …
Everything you need (and want) to know about tardigrades
WEBOct 18, 2022 · Collect a clump of moss or lichen (dry or wet) and place in a shallow dish, such as a Petri dish. Soak in water (preferably rainwater or distilled water) for 3-24 hours. Remove and discard excess water from the dish. Shake or squeeze the moss/lichen clumps over another transparent dish to collect trapped water.
DNA Sequencing: How to Choose the Right Technology
WEBAug 6, 2021 · The global market for DNA sequencing is predicted to grow from $15.7 billion in 2021 to $37.7 billion by 2026. The rising prevalence of viral diseases, such as COVID-19, and the increasing cases of cancer globally are likely to drive genomic research and propel the industry at an even more rapid rate.
DNA amplification techniques - Front Line Genomics
WEBAug 6, 2021 · DNA nanoball generation: Step #1: The DNA is fragmented and adapter sequences are attached. Step #2: A splint oligonucleotide hybridizes to the ends of the fragments, causing the DNA to form a circle. Step #3: The remaining linear DNA strands are removed, leaving only completed circular single-stranded DNA templates. Step #4: The circular DNA is replicated by phi29 DNA polymerase and the newly ...
A brief history of Next Generation Sequencing (NGS)
WEBJul 26, 2021 · 2000: Lynx Therapeutics Company launched the first of the NGS technologies, called Massively parallel signature sequencing (MPSS). The company was later bought by Illumina. 2004: 454 Life Sciences marketed a new generation pyrosequencing technology, called the Roche GS20. It …
A longstanding mystery: How do identical twins arise?
WEBOct 1, 2021 · Scientists have discovered DNA methylation differences that are unique to identical twins, helping to unravel the exact molecular events that lead to division of a zygote.. Monozygotic twins, or identical twins, result from the fertilisation of a single egg with a single sperm.At some point during the very early pre-implantation stages of development, the zygote divides into two embryos.
The evolution of DNA sequencing technologies
WEBFeb 12, 2021 · At the time, this technique was revolutionary. It enabled sequencing of 500-1,000bp fragments. A variant of the Sanger method – plus and minus method – developed by Sanger and Alan Coulson led to the first sequence of a DNA genome, that of bacteriophage φX174, in 1977. Two years later Allan Maxam and Walter Gilbert published their chemical ...
The Rarest of the Rare - Front Line Genomics
WEBFeb 23, 2021 · The Rarest of the Rare. In Europe, a rare disease is defined rare when it affects fewer than 1 in 2,000 people. However, this definition differs across the world. There are over 7,000 known rare diseases, many of which appear early in life. Unfortunately, about 30% of children with rare diseases will die before reaching their fifth birthday.